I. Soil ammonium nitrogen

(Indophenol blue method)

(1) Preparation of potassium chloride solution from soil extract: Remove a bag of potassium chloride from the reagent box and dissolve in 200 ml of distilled water.

(B) Determination procedures:

1: Weigh 5g of dried soil samples with a diameter of 2mm into a flask or a plastic bottle, add 25ml of soil extractant potassium chloride solution, shake it by hand for 20 minutes, and filter it on a small clean paper with filter paper. Beaker. This solution is the test solution.

2: Aspirate 5 ml of soil extractant potassium chloride solution into a clean, small beaker as a blank solution.

3: Pipette 5 ml of soil test solution into another clean small beaker.

4: Draw 5 ml of 4 ppm ammonium standard solution and put it in another clean beaker.

5: Add 5 ml of phenol solution and 10 ml of sodium hypochlorite to each of the above beakers respectively, 20 ml of distilled water, and shake and stand for 30 minutes.

6: Upper instrument colorimetric measurement: For details, see the instrument's use method in the instruction manual.

(c) Calculation of results:

Soil Ammonium Nitrogen (ppm) = Measured Fluid Content (C Value) x Liquid to Soil Ratio (Liquid Soil Ratio 5)

Second, the soil available phosphorus

(Molybdate)

(A) 1: Preparation of the soil extractant sodium bicarbonate solution: Remove a bag of sodium bicarbonate from the reagent box and dissolve in 200 ml distilled water.

2: Preparation of molybdenum sulfate anti-developer: 1.5 grams of ascorbic acid is dissolved in 100 ml of molybdenum sulfate solution.

(Note: This liquid is not easy to save. It will be prepared on the day of use.)

(B) Determination procedures:

1: Weigh 2 grams of air-dried soil sample with a diameter of 2 mm into a flask or a plastic bottle, add 40 ml of sodium bicarbonate solution, and shake it by hand at 25±1 degrees Celsius for 30 minutes. Use filter paper. Filter in a clean small beaker. This filtrate is the test solution.

2: Aspirate 5 ml of soil extractive sodium bicarbonate solution into a clean small beaker as a blank solution.

3: Pipette 5 ml of soil test solution into another clean small beaker.

4: Pipette 5 ml of 1 ppm of the available phosphorus standard solution into a clean small beaker.

5: Add 5 ml of molybdenum sulfate anti-developer to each beaker, remove the gas, add 40 ml of distilled water and shake for 30 minutes.

6: Upper instrument colorimetric measurement: For details, see the usage of the instrument in the instruction manual.

(c) Calculation of results:

Soil effective scale (ppm) = measured fluid content (C value) × liquid to soil ratio (liquid to soil ratio 20)

Three, soil available potassium

(turbidity method)

(1) Preparation of Sodium Nitrate Solution for Extracting Soil: Remove a bag of sodium nitrate from the reagent box and dissolve in 200 ml of distilled water.

(B) Determination procedures:

1: Weigh 6 grams of air-dried soil sample with a diameter of 2 mm into a flask or a plastic bottle, add 30 ml of soil extractive sodium nitrate solution, shake it by hand for 30 minutes, and filter it in a clean small beaker with filter paper. in. This filtrate is the test solution.

2: Aspirate 10 ml of soil extractant into a small clean beaker as a blank solution.

3: Draw 10 ml of soil test solution into another clean beaker.

4: Draw 10ml of 20ppm of available potassium standard solution and put it into another clean small beaker.

5: Add 10 drops of disodium EDTA solution, 2 ml of formaldehyde solution, and 2 drops of thymolphthalein indicator to each beaker, and then add 2 ml of sodium tetraphenylborate solution and 40 ml of distilled water to each beaker. Park for 5 to 10 minutes.

6: Upper instrument colorimetric measurement: For details, see the instrument's use method in the instruction manual.

(c) Calculation of results:

Soil available potassium (ppm) = measured fluid content (C value) × liquid to soil ratio during leaching (liquid to soil ratio is 10)

Fourth, soil organic matter colorimetry

(hydraulic heat-oxidation reaction method)

(a) Determination procedures:

1: Pipette 3.0 ml of distilled water into a clean small beaker and make a blank.

2: Weigh 1 g (accurate to 0.001 g) of air-dried soil sample with 0.5 mm pore size into another clean small beaker and add 3 ml of distilled water to fully shake the soil sample.

3: Aspirate 2 mL of standard solution of soil organic matter and put it in another clean small beaker. Add 1 mL of distilled water and the content is 10 mg.

4: Add 10 ml of potassium dichromate solution and 10 ml of concentrated sulfuric acid to each of the above small beakers. Shake constantly. Hold for 20 minutes and then add 10 ml of distilled water. Shake well. Re-precipitation (Note: Conditional The user can centrifuge the mixture in a small beaker with soil.

5: Pipette 10 ml of each of the solutions in the above small beakers (a small beaker with a soil sample must be pipetted with a clear solution), place in a 50 ml volumetric flask, and then dilute to the mark with distilled water and shake.

6: Upper instrument colorimetric measurement: For details, see the instrument's use method in the instruction manual.

(b) Calculation of results:

The soil organic matter content is expressed as a percentage and two decimal places are reserved.

Soil organic matter (%)=[(C×1.724)/(m×10 )]×100×1.32

=(C/m)×0.23

In the formula: C - the measured liquid content m - soil quality (1 g)

Hangzhou Top Instrument Co., Ltd.

No.11 West Park Road, West Lake Science Park, Hangzhou Tel 86062776

fax

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